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rabbit anti mouse tlr4 polyclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit anti mouse tlr4 polyclonal antibody
    Figure 3. Effects of berberine on key effectors of the <t>TLR4/NF-jB</t> signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
    Rabbit Anti Mouse Tlr4 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 3874 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+mouse+tlr4+polyclonal+antibody/rabbit+anti-mouse+IgG-B/pm33539718-70-13-19
    Average 96 stars, based on 3874 article reviews
    rabbit anti mouse tlr4 polyclonal antibody - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats."

    Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats.

    Journal: Pharmaceutical biology

    doi: 10.1080/13880209.2021.1877736

    Figure 3. Effects of berberine on key effectors of the TLR4/NF-jB signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
    Figure Legend Snippet: Figure 3. Effects of berberine on key effectors of the TLR4/NF-jB signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.

    Techniques Used: Expressing, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Comparison

    Related Articles

    Incubation:

    Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats
    Article Snippet: Total protein was separated by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% skim milk, followed by successive incubations with rabbit anti-mouse TLR4 polyclonal antibody (1:2000, Santa Cruz, USA) at 4 °C overnight and mouse anti-rabbit IgG monoclonal secondary antibody (1:10000, Bioss, China) for 2 h. The membranes were next incubated with ECL ultra western HRP substrate (Millipore, Billerica, MA, USA) and detected on a gel imaging system (ImageQuant LAS 4000, GE Healthcare, USA). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (CST, USA, diluted, 1:1000) was used as a reference control.

    Western Blot:

    Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats
    Article Snippet: Total protein was separated by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% skim milk, followed by successive incubations with rabbit anti-mouse TLR4 polyclonal antibody (1:2000, Santa Cruz, USA) at 4 °C overnight and mouse anti-rabbit IgG monoclonal secondary antibody (1:10000, Bioss, China) for 2 h. The membranes were next incubated with ECL ultra western HRP substrate (Millipore, Billerica, MA, USA) and detected on a gel imaging system (ImageQuant LAS 4000, GE Healthcare, USA). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (CST, USA, diluted, 1:1000) was used as a reference control.

    Imaging:

    Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats
    Article Snippet: Total protein was separated by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and transferred onto a polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, USA). .. The membranes were blocked with 5% skim milk, followed by successive incubations with rabbit anti-mouse TLR4 polyclonal antibody (1:2000, Santa Cruz, USA) at 4 °C overnight and mouse anti-rabbit IgG monoclonal secondary antibody (1:10000, Bioss, China) for 2 h. The membranes were next incubated with ECL ultra western HRP substrate (Millipore, Billerica, MA, USA) and detected on a gel imaging system (ImageQuant LAS 4000, GE Healthcare, USA). .. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (CST, USA, diluted, 1:1000) was used as a reference control.



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    Figure 3. Effects of berberine on key effectors of the <t>TLR4/NF-jB</t> signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
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    Figure 3. Effects of berberine on key effectors of the <t>TLR4/NF-jB</t> signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
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    Figure 3. Effects of berberine on key effectors of the <t>TLR4/NF-jB</t> signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.
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    Image Search Results


    Figure 3. Effects of berberine on key effectors of the TLR4/NF-jB signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.

    Journal: Pharmaceutical biology

    Article Title: Berberine attenuates septic cardiomyopathy by inhibiting TLR4/NF-κB signalling in rats.

    doi: 10.1080/13880209.2021.1877736

    Figure Lengend Snippet: Figure 3. Effects of berberine on key effectors of the TLR4/NF-jB signalling pathway. (A) TLR4 protein expression in the myocardial tissue and quantitation. (B) Nuclear p65 protein expression in the rat sepsis cardiomyopathy model measured by ELISA. Effects of berberine on TNFa (C) and IL 1b (D) levels in the myocardial tis- sue. p < 0.001 indicates significance in comparison with the Con group. p < 0.001 indicates significance in comparison with the LPS group.

    Article Snippet: The membranes were blocked with 5% skim milk, followed by successive incubations with rabbit anti-mouse TLR4 polyclonal antibody (1:2000, Santa Cruz, USA) at 4 C overnight and mouse anti-rabbit IgG monoclonal secondary antibody (1:10000, Bioss, China) for 2h.

    Techniques: Expressing, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Comparison